ORIGINAL_ARTICLE
Effects of Local Transplantation of Autologous Bone Marrow Mesenchymal Stem Cells in Combination with Low Level Laser Therapy in Repair of Experimental Acute Spinal Cord Injury in Rats
Objective- The aim of this study was to demonstrate the efficacy MSCs transplantation in combination with low level laser irradiation (low level laser irradiation) in repair of experimental acute spinal cord injury.Design- Experimental study.Animals- 28 adult male Wistar Rats.Procedures- A ballon- compression technique was used to produce an injury at the T8-T9 level of spinal cord applying Fogarty embolectomy catheter. In group-1, the autologous MSCs were transplanted to the spinal cord lesion; and followed by treatment with low level laser irradiation during 15 consecutive days in group-2. The injured rats in third group were treated by LLLI alone. The functional recovery was assessed using the Basso-Beattie-Bresnahan (BBB) locomotion scoring along 5 weeks.Results-In these three treatment groups, the score was significantly higher than control group. The differences among group-2 and two other treatment groups were statistically significant during all five weeks after treatment. There were no significant differences in BBB score between group-1(MSCs) and group-3(LLLI) at 3rd, 4th and 5th weeks of treatment. According to histopathological findings, the best response was observed in group-2(MSCs+LLLI) that repair of injured parts of dorsal funiculi and less cavitation were occurred by proliferation of mesenchymal stem cells and their differentiation to glial cells especially oligodendrocytes resulting in axon regeneration and relatively spinal cord recovery.Conclusion and Clinical Relevance- The findings of present study, demonstrate that concurrent use of LLLI and local transplantation of MSCs exhibits profound effects on axon regeneration and revealed remarkable functional improvement. These results suggest that MSCs characteristics could be influenced by low level laser irradiation, so this treatment may be as a useful procedure for neural regeneration, although further detailed investigations needs to be carried out particularly in clinical cases.
https://www.ivsajournals.com/article_51719_b87a6dc26a4b7da8cead6bad83473f06.pdf
2017-10-01
1
10
10.22034/ivsa.2017.51719
Spinal cord injury
Mesenchymal Stem Cells
Low level laser therapy
Seyed Sadra
Izadi
sadraizadi@gmail.com
1
Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
AUTHOR
Mohammad Mehdi
Dehghan
mdehghan@ut.ac.ir
2
Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
LEAD_AUTHOR
Davood
Sharifi
dsharifi@ut.ac.ir
3
Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
AUTHOR
Seyed Mehdi
Nassiri
nasirim@ut.ac.ir
4
Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
AUTHOR
Seyed Hossein
Mardjanmehr
hmehr@ut.ac.ir
5
Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
AUTHOR
Maryam
Zabihi
maryam.zabihi@rouginedarou.com
6
Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
AUTHOR
Javad
Ashrafihelan
ashrafihelan@yahoo.com
7
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
AUTHOR
Hesam
Akbarein
h.akbarein@gmail.com
8
Department of Epidemiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
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47
ORIGINAL_ARTICLE
In Vitro Evaluation of Equine Fibroblast-Like Synoviocytes Viability Treated with Doxycycline
Objective- The purpose of present study was to investigate the viability of equine fibroblast-like synoviocytes (FLSs) treated with doxycycline.Design- Experimental study.Sample population- FLSs from metacarpophalangeal joints of six skeletally mature horses.Methods- FLSs were established from synovial fluids of healthy joints. The cells were treated with different concentrations (1, 5, 10, 50, 100, 150, 300, 400 µg/ml) or without doxycycline for 48-hour. Viability of FLSs was determined by MTT assay and the Trypan blue dye exclusion method.Results- No significant differences were observed between viability of FLSs cultures treated with doxycycline until 150 µg/ml and control group (P>0.05). Doxycycline at 300 and 400 µg/ml significantly decreased FLSs viability (P<0.05). FLSs viability were 74.28% and 59.07% in 300 and 400 µg/ml, respectively, when measured by the MTT assay. Also FLSs viability at 300 and 400 µg/ml of doxycycline were 68.10% and 43.26%, respectively with Trypan blue exclusion method.Conclusion and Clinical Relevance- These findings demonstrate that doxycycline was not toxic for equine FLSs at concentration equal or less than 150 µg/ml in vitro. Further studies are needed to investigate the safety, efficacy and detrimental effects of doxycycline in equine joints.
https://www.ivsajournals.com/article_50823_2dead6e226d813e8adf97f64c3e73a49.pdf
2017-10-01
11
17
10.22034/ivsa.2017.50823
Doxycycline
Equine fibroblast-like synoviocytes
MTT assay
Trypn blue
viability
Samaneh
Ghasemi
samanehghasemi0@yahoo.com
1
Department of Clinical Sciences, School Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
AUTHOR
Kamran
Sardari
sardari@um.ac.ir
2
Department of Clinical Sciences, School Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
LEAD_AUTHOR
Pezhman
Mirshokraei
mirshokraei@um.ac.ir
3
Center of Excellence in Ruminant Abortion and Neonatal Mortality, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Razavi Khorasan, Iran
AUTHOR
Hossein
Hassanpour
hassanpourh@yahoo.com
4
Department of Basic Sciences (Physiology Division), Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Chaharmahal and Bakhtiari, Iran
AUTHOR
Mcllwraiht CW, Frisible DD and Kawcak CE. The horse as a model of naturally occurring osteoarthritis. Bone & Joint Research, 2012; 1: 279-309.
1
Monemdjou R, Fahmi H and Kapoor M. Synovium in the pathophysiology of osteoarthritis. Therapy, 2010; 7: 661-668.
2
Schlueter AE and Orth MW. Equine osteoarthritis: a brief review of the disease and its causes. Equine and Comparative Exercise Physiology, 2004; 1: 221-231.
3
Wang M, Sampson ER, Jin H, Li J, Ke QH, Im HJ and Chen DE. MMP13 is a critical target gene during the progression of osteoarthritis. Arthritis Research & Therapy, 2013; 15 (1): R5.
4
Burrage PS, Mix KS, and Brinckerhoff CE. Matrix metalloproteinases: role in arthritis. Frontiers in Bioscience, 2006; 11: 529-543.
5
Murphy G, Knäuper V, Atkinson S, Butler G, English W, Hutton M, Stracke J and Clark I. Matrix metalloproteinases in arthritic disease. Arthritis Research & Therapy, 2002; 4: S39-S49.
6
Manferdini C, Paolella F, Gabusi E, Silvestri Y, Gambari L, Cattini L, Filardo G, Fleury-Cappellesso S and Lisignoli G. From osteoarthritic synovium to synovial-derived cells characterization: synovial macrophages are key effector cells. Arthritis Research & Therapy, 2016; 18: 83.
7
Zrimšek PV, Kadunc Kos V, Mrkun J, and Kosec M. Diagnostic value of MMP-2 and MMP-9 in synovial fluid for identifying osteoarthritis in the distal interphalangeal joint in horses. Acta Veterinaria Brno, 2007; 76: 87-95.
8
Lu HT, Sheu MT, Lin YF, Lan J, Chin YP, Hsieh MS, Cheng CW and Chen CH. Injectable hyaluronic-acid-doxycycline hydrogel therapy in experimental rabbit osteoarthritis. BMC Veterinary Research, 2013; 9 (69).
9
Sokolove J and Lepus CM. Role of inflammation in the pathogenesis of osteoarthritis: latest findings and interpretations. Therapeutic Advances in Musculoskeletal Disease, 2013; 5: 77-94.
10
Mobasheri A. The future of osteoarthritis therapeutics: targeted pharmacological. Therapy, 2013; 15: 364.
11
Rogerson FM. Cartilage matrix degradation: an appropriate therapeutic target in osteoarthritis. Therapy, 2010; 7: 579-582.
12
Gerald N, Smith JR and Karen AH. Structure/function studies of doxycycline effects on matrix metalloproteinase activity and cartilage degeneration. In: Nelson M, Hillen W, Greenwald RA eds. Tetracyclines in Biology, Chemistry and Medicine. 1nd ed. Basel: Birkhäuser, 2001; 471-476.
13
Schnabel LV, Papich MG, Watts AE and Fortier LA. Orally administered doxycycline accumulates in synovial fluid compared to plasma. Equine Veterinary Journal, 2010; 42: 208-212.
14
Evans CH, Kraus VB, and Setton LA. Progress in intra-articular therapy. Nature Reviews Rheumatology, 2014; 10: 11-22.
15
Haerdi-Landerer MC, Suter MM, and Steiner A. Intra-articular administration of doxycycline in calves. American Journal of Veterinary Research, 2007; 68: 1324-1331.
16
Sherman, S.L., James, C., Stoker, A.M., Cook, C.R., Khazai, R.S., Flood, D.L., and Cook, J.L. 2015. In vivo toxicity of local anesthetics and corticosteroids on chondrocyte and synoviocyte viability and metabolism. Cartilage, 6: 106-112.
17
Khademhosseini A. MTT Cell Assay. Khademhosseini Lab. Protocol Manual. 2006; 92-94.
18
Munari CC, de Oliveira PF, de Souza Lima IM, de Paula Lima Martins S, de Carvalho da Costa J, Bastos JK, and Tavares DC. Evaluation of cytotoxic, genotoxic and antigenotoxic potential of Solanum lycocarpum fruits glicoalkaloid extract in V79 cells. Food and Chemical Toxicology, 2012; 50: 3696-3701.
19
Di Caprio R, Lembo S, Di Costanzo L, Balato A, and Monfrecola G. Anti-inflammatory properties of low and high doxycycline doses: an in vitro study. Mediators of Inflammation, 2015; 1-10.
20
Leite LM, Carvalho AG, Ferreira PL, Pessoa IX, Gonçalves DO, Lopes Ade A, Góes JG, Alves VC, Leal LK, Brito GA and Viana GS. Anti-inflammatory properties of doxycycline and Minocycline in experimental models: an in vivo and in vitro comparative study. Inflammopharmacology, 2011; 19: 99-110.
21
Verbruggen G. Chondroprotective drugs in degenerative joint diseases. Rheumatology (Oxford), 2006; 45: 129-138.
22
Karna E, Pałka J, Wołczyński S. Doxycycline-induced inhibition of prolidase activity in human skin fibroblasts and its involvement in impaired collagen biosynthesis. European Journal of Pharmacology, 2001; 430: 25-31.
23
Myers SA and Wolowacz RG. Tetracycline-based MMP inhibitors can prevent fibroblast-mediated collagen gel contraction in vitro. Advances in Dental Research, 1998; 12: 86-93.
24
Soe JH, Ciu DZ and Kim YJ. Inhibiton of MMP-13mRNA expression by doxycycline combination with mefenamic Acid in the rat periodontal ligament cells (abstract in English). Journal of Korean Academy of Periodontology, 2005; 35: 99-109.
25
Suzuki A, Yagisawa J, Kumakura S and Tsutsui T. Effects of minocycline and doxycycline on cell survival and gene expression in human gingival and periodontal ligament cells. Journal of Periodontal Research, 2006; 41: 124-131.27.
26
Smith VA, Cook SD. Doxycycline-a role in ocular surface repair. British Journal of Ophthalmology, 2004; 88: 619-625.
27
ORIGINAL_ARTICLE
Anaesthetic Effect of Propofol on Rainbow Trout (Oncorhynchus Mykiss) in Two Different Concentrations
Objective- The study aims to determine efficacy of propofol as an inmersión agent to induce anesthesia in rainbow trout (Oncorhynchus mykiss).Design- Experimental study.Animals- 36 healthy rainbow troutProcedure- Trouts were sorted ramdomly in two groups, 18 fish each one. Both groups were anesthesized by bath, one of them with 2,5 mg/l, the other one at 5 mg/l concentration. During the experiment, basal respiratory rate, partial and total equilibrium loss, time to anesthesia, anaesthesia respiratory rate and manipulation response were recorded.Results and Conclusion- Induction and recovery times as well as behavioural response were recorded, being significantly affected by propofol concentration (P <0.01). After exposure to 2,5 and 5 mg/l, fishes reached stage 3 anaesthesia in 4,99 ± 1,07 and 2,81 ± 0,71 minutes respectively. Recovery time were 3,59 ± 1,44 for 2,5 mg/l and 7,49 ± 3,02 minutes for 5 mg/l. After the experiment, the fish remained for 48 hours in a pond attached to the unit, without any death. This study, showed the behavioural response of rainbow trout to anaesthesia as well effectiveness of propofol as anaesthetic. Propofol induce safe dose dependent anaesthesia, being useful for different tasks related to the management of culture trout, as it meets the criteria established in aquaculture use.Clinical relevance- The results of the present work provide data to be used in surgical procedures and containment maneuvers in the different practices performed in fish farming.
https://www.ivsajournals.com/article_51290_84cd5032b23ec25c5cbc6d4dda3dae79.pdf
2017-10-01
18
24
10.22034/ivsa.2017.51290
Propofol
rainbow trout
Anaesthesia
Guillermo
F. Prieto
gprieto@ayv.unrc.edu.ar
1
Department of Pharmacology, Faculty of Agronomy and Veterinary Medicine, Río Cuarto National University, Río Cuarto, Córdoba, República Argentina
LEAD_AUTHOR
Natalia
F. Urzua
nurzuapizarro@ayv.unrc.edu.ar
2
Department of Pharmacology, Faculty of Agronomy and Veterinary Medicine, Río Cuarto National University, Río Cuarto, Córdoba, República Argentina
AUTHOR
Miguel
A. Mancini
mmancini@ayv.unrc.edu.ar
3
Department of Aquaculture, Faculty of Agronomy and Veterinary Medicine, Río Cuarto National University, Río Cuarto, Córdoba, República Argentina
AUTHOR
Maria
P.Tonini
mptonini@ayv.unrc.edu.ar
4
Department of Pharmacology, Faculty of Agronomy and Veterinary Medicine, Río Cuarto National University, Río Cuarto, Córdoba, República Argentina
AUTHOR
Jimena
Messina
jimenamessina@hotmail.com
5
Department of Pharmacology, Faculty of Agronomy and Veterinary Medicine, Río Cuarto National University, Río Cuarto, Córdoba, República Argentina
AUTHOR
Sergio
Salas
piscicultura@hotmail.com
6
Boca de Río Fish Farm , Córdoba, República Argentina
AUTHOR
Carlos
A. Errecalde
7
Department of Pharmacology, Faculty of Agronomy and Veterinary Medicine, Río Cuarto National University, Río Cuarto, Córdoba, República Argentina
AUTHOR
Ugwemorubong U G and Akinrotimi O A. Management of stress in fish for sustainable aquaculture development. Researcher, 2011; 4: 28-38.
1
Husen A, Sharma A H. Efficacy of anesthetics for reducing stress in fish during aquaculture practices - a review. Kathmandu University Journal of Science, Engineering and Technology, 2014; 10: 104-123.
2
Charoendat U, Areechon N, Srisapoome P and Chantasart D. Efficacy of synthetic eugenol as an anesthetic for Nile tilapia (Oreochromis niloticus Linnaeus). Kasetsart Journal - Natural Science, 43: 132-140.
3
Neiffer D L and Stamper M A. Fish Sedation, anesthesia, analgesia, and euthanasia: considerations, methods, and types of drugs. ILAR Journal, 2009; 4: 343-360.
4
Coyle S, Durborow R and Tidwell J. Anesthetics in Aquaculture. SRAC Publication, 2004, 3900
5
Sneddon L U. Clinical anesthesia and analgesia in fish. Journal of Exotic Pet Medicine, 2012; 21: 32-43.
6
Zahl I H, Samuelsen O and Kiessling A. Anaesthesia of farmed fish: implications for welfare. Fish Physiology and Biochemistry, 2012; 38: 201-218.
7
Sladky K, Swanson C R, Stoskopf M, Loomis M R and Lewbart G A. Comparative efficacy of tricaine methane sulfonate and clove oil for use as anesthetics in Red pacu (Piaractus brachypomus). 3: 337-342.
8
Stoskopf M, Acuicultura para Veterinarios: Producción y clínica de peces. Anestesia. (Ed) Lydia Brown: Editorial ACRIBIA, A.S; 2000: 169-171.
9
Gomułka P, Wlasow T, Szczepkowski M, Misiewicz L and Ziomek E. The effect of propofol anaesthesia on haematological and biochemical blood profile of European whitefish. Turkish Journal of Fisheries and Aquatic Sciences, 2014; 14: 331-337.
10
Ostrensky A, Pedrazzani A S and Vicente A L. Use of MS-222 (tricaine methanesulfonate) and propofol (2,6-diisopropylphenol) as anaesthetics for the tetra Astyanax altiparanae (Teleostei, Characidae). Aquaculture Research, 2016; 47: 3477–3488.
11
Sawyer D. The Practice of Veterinary Anesthesia: Small Animals, Birds, Fish and Reptils. Jackson: Teton New Media Incorporated, 2008; 70-75.
12
Gholipourkanani K, Ahadizadeh S. Use of propofol as an anesthetic and its efficacy on some hematological values of ornamental fish Carassius auratus. Springerplus, 2013; 2: 76.
13
Valença-Silvaa G, Brazb M, Barretoa R, Salvadoric D and Volpatoa G. Low Dose of the Anesthetic Propofol Does Not Induce Genotoxic or Mutagenic Effects in Nile Tilapia. Transactions of the American Fisheries Society, 2014. 14:53.
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Oda A, Bailey K M, Lewbart G A, Griffith E H and Posner L P. Physiologic and biochemical assessments of koi (Cyprinus carpio) following immersion in propofol. Journal of the American Veterinary Medical Association, 2014; 245: 1286-1291
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Balko J, Wilson S, Lewbart G, Gaines B, and Posner L. Propofol as an immersion anesthetic and in a minimum anesthetic concentration (MAC) reduction model in goldfish (Carassius auratus). Journal of Zoo and Wildlife Medicine, 2017; 48(1): 48–54.
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FDA, Data sheet: DIPRIVAN® (propofol 1%) inyectable emulsion, USP. Reference ID: 4089428, revised 451094H. 2017.
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Gressler L, Sutili F, Teixeira da Costa S, Parodi T, da Silva Pes T, Koakoski G, Barcellos G and Baldisserotto, B. Hematological, morphological, biochemical and hydromineral responses in Rhamdia quelen sedated with propofol. Fish Physiology and Biochemistry, 2015; 41: 463-472.
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Delgado L and Schmachtenberg O. Immunohistochemical localization of GABA, GAD65, and the receptor subunits GABAAa1 and GABAB1 in the zebrafish cerebellum. Cerebellum. 2008;7(3):444–450.
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Peyghan R, Papahn A A and Nadaf H, Ebadi A. Anesthesia with Propofol in Grass Carp, Ctenopharyngodon idella, and its effects on electrocardiogram, blood bases and pH. Iranian Journal of Veterinary Surgery, 2008; 3: 9-18.
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Ross LG and Ross B. Anaesthetic and sedative techniques for aquatic animals. 3rd ed. Oxford: Blackwell Publishing, 2008; 69-126.
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Treves-Brown K M. Applied fish pharmacology. Dordrecht: Springer Science+Business Media BV, 2000; 206-218.
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Velisek J, Wlasow T, Gomulka P, Svobodova Z and Novotny L. Effects of 2-phenoxyethanol anaesthesia on Sheatfish (Silurus glanis L.). Veterinární Medicína, 2007; 52: 103-110.
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Blanco Cachafeiro M C. La trucha, cría industrial, Ediciones Mundi-Prensa, Madrid, 1984; 17-46.
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Mendoza-Bojorquez R J, Palomino-Ramos AR. Manual de cría de truchas arco iris en jaulas flotantes, AECI/PADESPA, España, 2004; 31-35.
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Llanos C and Scotto C. Eugenol como anestésico para labores de manipulación de Xiphophorus helleri (Heckel, 1848) (Cyprinodontiformes: Poecilidae), The Biologist (Lima), 2010; 8:179-188
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Prieto G, Errecalde C, Mancini M, Urzúa N, Tonini M and Salas S. Valoración de la actividad depresora de diferentes concentraciones de eugenol en trucha arco iris (Oncorhynchus mykiss). Revista Medicina Veterinaria (Buenos Aires), 2015; 96: 21-25.
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Keene J, Noakes D, Moccia R and Soto C. The efficacy of clove oil as an anaesthetic for rainbow trout, Oncorhynchus mykiss (Walbaum). Aquaculture Research, 1998; 29(2), 89-101.
29
Blanco Cachafeiro M C. La trucha, cría
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industrial, Ediciones Mundi-Prensa, Madrid, 1984; 17-46.
31
ORIGINAL_ARTICLE
Beneficial Effects of Ag-Exchanged Zeolite Nanocomposite on Excisional Wound in Rats
Objective-The aim of this study was to investigate the healing effects of Ag+- zeolite/gelatin nanocomposite on excisional wound healing in rat animal model.Design-Experimental studyAnimals-Eighteen male Sprague-Dawly rats weighing 200-220gProcedure- Ag+- zeolite/gelatin nanocomposite was fabricated by sol-gel method, and characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD) techniques. MTT assay and antimicrobial activity evaluation of the nanocomposite were performed. Under general anesthesia, a full thickness wound measuring 1.5×1.5 cm was created on dorsal area of each rat. The animals were equally and randomly divided into three groups of 6 each i.e. group I (0.9% sodium chloride), group II (gelatin –treated) and group III (nanocomposite –treated). The solutions and the formulation were applied topically on the wound once daily for 14 days. Photograph of each wound was taken on days 0,3,6,9,12 and 14 post wound creation. The area of wound was determined planimetrically. At 14 days, animals euthanized and skin samples were taken to histopathologicl evaluation (H&E staining).Results- In this work, we successfully prepared Ag+- zeolite/gelatin nanocomposite. The prepared nanocomposite showed antimicrobial activity due to Ag ion-exchanging. The results indicate nanocomposite is safe up to 0.1 mg/ml of Ag+- zeolite/gelatin nanocomposite. Nanocomposite treated group exhibited enchantment of wound closure and accelerate wound healing time (p<0.05). Furthermore, nanocomposite treated group showed higher neovascularization and collagen content and faster regrowth of epidermis in repair area compared to other groups.Conclusion and clinical relevance- In conclusion, biocompatible Ag+- zeolite/gelatin nanocomposite might have great application for open and full thickness wound healing.
https://www.ivsajournals.com/article_50288_dabe78367b58a8f899b1cfc6770571de.pdf
2017-10-01
25
32
10.22034/ivsa.2017.50288
Nanocomposite
Ag+- zeolite/gelatin
Excisional wound
Rat
Sara
Javanmardi
sarahjavanmardi@yahoo.com
1
Department of Clinical Sciences, Faculty of Veterinary medicine, University of Tabriz, Tabriz, Iran.
LEAD_AUTHOR
Baharak
Divband
2
Department of Inorganic chemistry, University of Tabriz, Tabriz, Iran.
AUTHOR
Heather L.O, David K, Louise F, Marie F. Basic principles of wound healing. Journal of Wound Care Canada, 2004;9:1-12.
1
Tocco I, Zavan B, Bassetto F, Vindigni V. Nanotechnology-based therapies for skin wound regeneration. Journal of Nanomaterials, 2012;4:7-11
2
Neethu N, Muthunarayanan M, Nur A, In-Kyu P, Anne E, Tin W, Sabu T, Yves G. Antibacterial and wound healing analysis of gelatin/zeolite scaffolds. Journal of Colloids and Surface B: Biointerface,2014;115:224-252.
3
grancaric A.M, Tarbuk A, Kovacek I. Nanoparticle of activated natural zeolite on textile for protection and therapy. Journal of Chemical Engineering Quartely, 2009;15:203-210.
4
Ninan N, Muthian m, Park IK, Elain A, Thomas S, Grohens Y. Pectin/carboxymethyl cellulose/microfibrillated cellulose composite scaffolds for tissue engineering. Journal of Carbohydrate polymer,2013:98:877-885.
5
Jayakumar R, Prabaharan M, Kumar P, Nair SV, Tamura H. Biomaterials based on chitin and chitosan in wound dressing applications. Journal of Biotechnology advances,2010;29:322-337.
6
Tianhong D, masamitu T, Huang Y, Hambin M. Chitosan preparation for wounds and burns:antimicrobial and wound-healing effects. Journal of Expert Review of Anti-infective Therapy, 2011;9:857-879.
7
Steven l, Sara M. silver and alginates: Role in wound healing and biofilm control. Journal of Advances in Wound Care (New Rochelle), 2015;4(7):407-414.
8
Tanaka A, Nagate T, Matsuda H. Acceleration of wound healing by gelatin film dressings with epidermal growth factor. Journal of Veterinary Medicine Science, 2005;67:909-13.
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wang j, Wang Z, Zhang S, Song Y, Dong X, Wang J. Antibacterial and anti-adhesion zeolite coatings on titanium alloy surface. Journal of Microporous Mesoporous Materials, 2011;146:216-222.
10
Breuing K, Andree G, Helo J, Slama P.Y, Liu E. Growth factors in the repair of partial thickness porcine skin wounds. Journal of Plastic Reconstructive & aesthetic Surgery,1997;100:657-664.
11
Joshua S, Matthews KH, Howard N.E, Gillian M. Woun healing dressings and delivery systems: A review. Journal of pharmaceutical Sciences, 2008;97:2892-2923
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Gong CY, Wu Q, Wang Y, Zhang D, Luo F, Zhao X, wei Y, Qian Z. A biodegradable hydrogel system containing curcumin encapsulated in micelles for cutaneous wound healing. Journal of Biomaterials,2013;34:6377-6387.
13
Abbasalipour M, Mirjalili M, Khajavi R, Majidi M. Coated cotton gauze with Ag/ZnO/chitosan nanocomposite as a modern wound dressing. Journal of Engineered Fibers and Fabrics, 2014;9:124-130.
14
El-refaie W, Elnarggar Y, El-Massik M, Abdallah O. Novel curcumin-loaded gel-core hyaluosomes with promising burn-wound healing potential:Development, in-vitro appraisal and in-vivo studies. International Journal of pharmaceutids, 2015;486:88-98.
15
Young A, Mcnaught C. The physiology of wound healing. Journal of Surgery,2011;29-475-79.
16
Gopal A, Kant V, Gopalakrishnan A, Tandan S, Kumar D. Chitosan-based copper nanocomposite accelerates healing in excision wound model in rats. European Journal of Pharmacology, 2104;731:8-19.
17
Archana D, Sigh b, Dutta J, Dutta P.K. Chitosan-PVP-nanosilver oxide wound dressing: In vitro and in vivo evaluation. International Journal of Biological macromolecules ,2015;73:49-57.
18
Jiang L, Yan W, Xueyong L, Yuejun L, Wangzhon L, Shoozong C. Addition of an alginate to a modified zeolite improves hemostatic performance in a swine model of lethal groin injury. Journal of Trauma Injurry, Infection and Critical Care, 2009;66:612-620.
19
Cutting K, Rogers AA, Rippon M. The importance of hydration in wound healing : re invigorating the clinical prespective. Journal of wound care, 2016; 25:122-124
20
Kang B, Cheon Y, Jin Y. Comparision of the wound healing effect of cellulose and gelatin: An in vivo study. Journal of Archives of Plastic Surgery, 2012;39:317-322.
21
Bum Sik Kang, Young Cheon Na, Young Wan Jin.Comparision of the wound healing effect of cellulose and gelatin:An in vivo study. Journal of Archives of Plastic Surgery, 2012;39: 317-22.
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Bainbrifge P. Wound healing and the role of fibroblasts. Journal of wound care, 2013;22:407-412.
23
Naraginti S, Kumari P, Das R, Sivakumar A, Patil S, Andhalkar V. Amelioration of excision wounds by topical application of green synthesized, formation silver and gold nanoparticles in albino wistar rats. Journal of Materials Science and Engineering,2016;c62:293-300.
24
Archana D, Singh B, Dutta J, Dutta P.K. Chitosan-PVP-Nano silver oxide wound dressing: In vitro and in vivo evaluation. International Journal of Biological Macromolecules, 2015;73:49-57.
25
Krauza A, Alder B, Cabral V, Navati M, Doerner J, Charafeddine R, Chandra D, Liang H, Gunther L, Clendaniel A, Harper S, Friedman J, Nosanchuk J, Friedman A. Curcumin-encapsulated nanoparticles as innovative antimicrobial and wound healing agent. Journal of Nanomedicine:Nanothecnology, Biology and Medicine, 2015; 11:195-206.
26
Sabine A Eming , bent Brachvogel , Tresa Odorisio, Manuel Koch. Regulation of angiogenesis: Wound healijng as a model. Journal of Progress in histochemistry and cytochemistry, 2007;42:115-170
27
ORIGINAL_ARTICLE
Comparison of Autogenic Costal Cartilage with Chitosan Scaffold in Canine Humeral Defect Healing
Objective- Current trends emphasize the acceleration of fracture healing on the ground that in doing so, the limitation of mobility and complications associated with recovery period are reduced. The present study aims to compare autogenic costal cartilage with Chitosan scaffold in canine humeral defect healing. Design- Experimental studyAnimal-15 adult male dogs Procedures- Dogs were divided into three groups of five. Humerus window shaped defect was created in their right hands. In the first group (controls), the defect was left untreated. In the second and third groups, Chitosan and autogenic costal cartilage were placed into the defects, respectively. Radiographs of the defects were prepared at weeks 2, 4, 6 and 8 and finally the dogs were euthanized after 70 days. Histological sections were also obtained from the defect sites.Result-The results indicated that the costal cartilage alone treated group was inferior to both Chitosan treated and control groups, so cartilage does not seem to serve as a suitable alternative for grafting in canine bone defects. Conclusion and Clinical Relevance- Taking into account the results and other recent reports, it can be concluded that chitosan scaffolds with greater capabilities can be used in canine bone defect healing, however, for ideal bone tissue regeneration, chitosan as a base has to be combined with other materials including those mentioned above. The present study results showed that cartilage cannot serve as a proper alternative for grafting. Keywords: Autogenic Costal cartilage, Chitosan Scaffold, Bone Defect, Canine
https://www.ivsajournals.com/article_50822_63f678bce58b2f8b96aa3d1b8f331b30.pdf
2017-10-01
33
39
10.22034/ivsa.2017.50822
Autogenic Costal cartilage
Chitosan Scaffold
Bone Defect
Canine
Siavash
Sharifi
drsharifisiavash94@gmail.com
1
Department of Clinical Sciences, College of Veterinary Medicine, University of ShahreKord, ShahreKord, Iran.
LEAD_AUTHOR
Iraj
Karimi
irkarimi@yahoo.com
2
Department of Pathobiology, College of Veterinary Medicine, University of ShahreKord, ShahreKord, Iran.
AUTHOR
Saeed
Soltani
saeedsoltany36@yahoo.com
3
Department of Clinical Sciences, College of Veterinary Medicine, University of ShahreKord, ShahreKord, Iran.
AUTHOR
Amin
Bigham-Sadegh
dr.bigham@gmail.com
4
Department of Clinical Sciences, College of Veterinary Medicine, University of ShahreKord, ShahreKord, Iran.
AUTHOR
Farzaneh
Hosseini
hosseinifm@gmail.com
5
Department of Clinical Sciences, College of Veterinary Medicine, University of ShahreKord, ShahreKord, Iran.
AUTHOR
Lacroix D, Prendergast P. A mechano-regulation model for tissue differentiation during fracture healing: analysis of gap size and loading. Journal of biomechanics 2002;35:1163-1171.
1
Bardsley K, Kwarciak A, Freeman C, Brook I, Hatton P, Crawford A. Repair of bone defects in vivo using tissue engineered hypertrophic cartilage grafts produced from nasal chondrocytes. Biomaterials 2017;112:313-323.
2
Damien CJ, Parsons JR. Bone graft and bone graft substitutes: a review of current technology and applications. Journal of Applied Biomaterials 1991;2:187-208.
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Arrington ED, Smith WJ, Chambers HG, Bucknell AL, Davino NA. Complications of iliac crest bone graft harvesting. Clinical orthopaedics and related research 1996;329:300-309.
4
Lee S-H, Shin H. Matrices and scaffolds for delivery of bioactive molecules in bone and cartilage tissue engineering. Advanced drug delivery reviews 2007;59:339-359.
5
Drewnowska O, Turek B, Carstanjen B, Gajewski Z. Chitosan–a promising biomaterial in veterinary medicine. Polish journal of veterinary sciences 2013;16:843-848.
6
Saravanan S, Leena R, Selvamurugan N. Chitosan based biocomposite scaffolds for bone tissue engineering. International journal of biological macromolecules 2016.
7
Pearce A, Richards R, Milz S, Schneider E, Pearce S. Animal models for implant biomaterial research in bone: a review.
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Lane JM, Sandhu H. Current approaches to experimental bone grafting. The Orthopedic clinics of North America 1987;18:213-225.
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Heiple K, Goldberg VM, Powell A, Bos G, Zika J. Biology of cancellous bone grafts. The Orthopedic clinics of North America 1987;18:179.
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Saifzadeh S, Hobbenaghi R, Hodi S. Elastic cartilage grafting in canine radial fracture. Iranian Journal of Veterinary Research 2006;7:1-7.
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Montufar-Solis D, Nguyen H, Nguyen H, Horn W, Cody D, Duke P. Using cartilage to repair bone: an alternative approach in tissue engineering. Annals of biomedical engineering 2004;32:504-509.
12
Pippenger BE, Ventura M, Pelttari K, Feliciano S, Jaquiery C, Scherberich A, Walboomers XF, Barbero A, Martin I. Bone‐forming capacity of adult human nasal chondrocytes. Journal of cellular and molecular medicine 2015;19:1390-1399.
13
Li X, Wang X, Zhao T, Gao B, Miao Y, Zhang D, Dong Y. Guided bone regeneration using chitosan-collagen membranes in dog dehiscence-type defect model. Journal of Oral and Maxillofacial Surgery 2014;72:304. e301-304. e314.
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Prabaharan M, Mano J. Chitosan-based particles as controlled drug delivery systems. Drug delivery 2004;12:41-57.
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Junqueira LC, Carneiro J. Basic histology text and atlas: London: McGraw Hill, 2005, 2005.
16
Sundaram MN, Deepthi S, Jayakumar R. Chitosan-Gelatin Composite Scaffolds in Bone Tissue Engineering. Chitin and Chitosan for Regenerative Medicine: Springer, 2016;99-121.
17
ORIGINAL_ARTICLE
Autologous Platelet Rich Plasma Injection Improves Early Tendon Repair in Rabbits: A Histopathological and Biomechanical Study
Objective- The aim of this study was to investigate the PRP effects on the early time-period during tendon healing in rabbits DDF tendon.Design-Experimental studyAnimals- Twenty male New Zealand white rabbitsProcedure-PRP samples were prepared using twice centrifugation method of modification of the Cuarsan technique. Animals were randomly assigned into two equal treatment and control groups. The injury model was unilateral complete transection through the middle one third of deep digital flexor tendon. Immediately after primary repair, either 0.5 cc PRP or placebo was injected intratendiously into the suture site in the treatment and control groups, respectively. Operated limbs were immobilized for two weeks. Animals were sacrificed at the third week and the tendons underwent histopathological (H&E and MT staining) and biomechanical evaluation.Results- The histopathological (H&E) observation showed significant increase in percentage of fibrillar linearity, fibrillar contiuity, number of capillaries in epitenon and epitenon thickness in PRP treated group compared to the control group (p<0.05). Results from MT staining revealed more collagen deposition, in treatment group in comparison to the control. Results of mechanical testing revealed the significant improvement of force at failure and energy absorption capacity of repaired in the PRP group in comparison to the control (p<0.05).Conclusion and clinical relevance-The present study findings suggest that PRP is a simple, safe, quick and cost effective way to obtain a natural concentration of autologous growth factors which reduce the risk of rupture after tendon primary repair and improve functional outcomes.
https://www.ivsajournals.com/article_50348_d03fa29c132862d8f40b7b83fb1b95d6.pdf
2017-10-01
40
48
10.22034/ivsa.2017.50348
Platelet rich plasma
DDF tendon
Rabbits
Sara
Javanmardi
sarahjavanmardi@yahoo.com
1
Department of Clinical Sciences, Faculty of Veterinary medicine, University of Tabriz, Tabriz, Iran.
LEAD_AUTHOR
Amirata
Vosough
2
Graduated of Veterinary Medicine, Faculty of Veterinary medicine, University of Tabriz, Tabriz, Iran.
AUTHOR
Raziallah
Jafari Jozani
3
Department of Clinical Sciences, Faculty of Veterinary medicine, University of Tabriz, Tabriz, Iran.
AUTHOR
Javad
Ashrafi-Helan
ashrafihelan@yahoo.com
4
Department of Pathobiology, Faculty of Veterinary medicine, University of Tabriz, Tabriz, Iran.
AUTHOR
Carpenter JE, Hankenson KD. Animal models of tendon and ligament injuries for tissue engineering applications. Journal of Biomaterial, 2004; 25:1715-22.
1
Lin LW, Cardenas L, Soslowsky LJ. Biomechanics of tendon injury and repair. Journal of Biomechanic, 2004; 37:865-77.
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Frank C, Donald D, Shrive N. Collagen fibril diameter in the rabbit medial collateral ligament scar: a longer term assessment. Journal of Connective Tissue Research, 1997; 36:21-9.
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Anitua E, Andia I, Ardanza B, Nurden P. Autologous platelet as a source of proteins for healing and tissue regeneration. Journal of Thrombosis and Haemostasis, 2004; 91:4-15.
4
Chan PB, Qin L, Lee KM, Rolf CG, Chan KM. Effect of fibroblast growth factor (bFGF) on early stages of tendon healing. Journal of Acta Orthopedic Scandinavia,1999; 71:513-18.
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Zhang F, Lineaweaver WC. Growth factors and gene transfer with DNA strand technique in tendon healing. Journal of Long Term Effects of Medical Implants, 2002; 12:105-112.
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Abrahamsson SO, Lundborg G, Lohmander LS. Recombinant human insulin-like growth Factor-I stimulates in vitro matrix synthesis and cell proliferation in rabbit flexor tendon. Journal of Orthopedic Research, 1991; 9:495-502.
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Dahlgren LA, Mohammed HO, Nixon AJ. Temporal expression of growth factor and matrix molecules in healing tendon lesion, Journal of Orthopedic Research 2005; 23:84-97.
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Kon E, Filardo G, Delcoglino M, Presti ML, Russo A, Bondi A. Platelet-rich plasma: new clinical application. A pilot study for treatment of jumper’s knee. Injury 2009;40(6):598-603.
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Robert EM. Platelet-rich plasma(PRP): what is PRP and what is not PRP. J Implant Dentistry 2001;10(4):225-8.
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Abbasalipour M, Mirjalili M, Khajavi R, Majidi M. Coated cotton gauze with Ag/ZnO/chitosan nanocomposite as a modern wound dressing. Journal of Engineered Fibers and Fabrics, 2014;9:124-130.
12
Weibrich G, Kleis WK, Hafner G, Hitzler WE, Wanger W. Comparision of platelet, leukocyte, and growth factor levels in point-of-care platelet enrich plasma, prepared using a modified Curasan kit, with preparations received from a local blood bank. Clinical Oral Implants Research, 2003;14(3)357-62
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Behfar M, Sarrafzadeh-Rezaei F, Hobbenaghi R, Delirezh N, Dalirnaghadeh B. Adipose derived stromal vascular fraction improves early tendon healing:an experimental study in rabbits. Journal of Veterinary Research Forum, 2011;2(4):248-53.
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Behfar M, Javanmardi S, Sarrafzadeh-Rezaei F. Comparative study on fictional effects of allotransplantation of bone marrow cells and adipose derived stromal vascular on tendon repair: A biomechanical study in stromal rabbits. Journal of Cell (Yakhteh),2014;16(3):263-70.
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Chan KM, Hui WC, Chan LS, Rui YF, Qin L, Hung LK. Radix dipsaci dose note improve tendon healing in a rat model of patellar tendon donor site injury. Journal of Orthopedic Surgery, 2010; 2:187-93.
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Lin TW, Cardenas L, Suslowsky LJ. Biomechanic of tendon injury and repair. Journal of Biomechanic,2004, 37:865-877.
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Luminita SL, Dan C. Clinical review about the role of platelet rich plasma for the treatment of traumatic and degenerative musculoskeletal disorders. Orthopedics and Rheumatology Open Access Journal, 2016;2(3):1-9.
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Gentile P, Cole JP, Cole M, Garcovich S, Bielli A, Scioli MG. Evaluation of Not-Activated PRP in hair loss treatment: Role of growth factor and cytokine concentrations obtained by different collection systems. International Journal of Molecular Science, 2017;18(2):408-422.
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Morizaki Y, Zhao C, Amadio PC. The effects of platelet-rich plasma on bone marrow stromal cell transplants for tendon healing in vitro. Journal of Hand Surgery,2010;35(11):1833-4.
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Timothy M, Yao W, Georg AC. The roles of growth factors in tendon and ligament healing. Journal of Sport Medicine, 2003;33(5):381-94.
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-Kakar S, Khan U, McGrouther DA. Differential cellular response within the rabbit tendon unit following tendon injury. Journal of Hand Surgery: British ,1998; 23:627-32.
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Matsui M, Tabata Y. Enhanced angiogenesis by multiple release of platelet-rich plama contents and basic fibroblast growth factor from gelatin hydrogels. Journal of Acta Biomaterial, 2012;8:1792-1801.
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Ferrara N. Role of vascular endothelial growth factor in the regulation of angiogenesis. Journal of Kidney International ,1999;56(3):794-814.
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Cross M, Claesson WL. FGF and VEGF function in angiogenesis: signaling pathways, biological responses and therapeutic inhibition. Journal of Trends in Pharmacological Sciences, 2001; 2(4):201-7.
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Docheva D, Muller SA, Majewska M, Evans CH. Biologics for tendon repair. Journal of Advanced Drug Delivery Reviews, 2015; 84:22-3
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Sharma P, Maffulli N. Biology of tendon injury: healing modeling and remodeling. Journal of Musculoskeletal and neuronal Interaction, 2006;6(2)181-190
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. Amiel D, Akeson W, Harwood FL, Frank CB. Stress deprivation effect on metabolic turnover of medial collateral ligament collagen. A comparison between nine-and 12-week immobilization. Journal of Clinical Orthopedic and Trauma, 1993; 172:265-70.
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Orhan K, Ozturan A, Guven CK. The effect of extracorporeal shock waves on a rat model of injury to tendo Achillis: ahistological and biomechanical study. Journal of Bone Joint Surgery: British, 2004;86-B:613-8.
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Wurgler-Hauri C, Dourte L M, Baradet T C,Williams GR, Soslowsky LJ. Temporal expression of eight growth factors in tendon to bone healing in a rat supraspinatus model. Journal of Shoulder and Elbow Surgery, 2007; 16(50): S198-S203.
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Aspenberg P, Vichenko O. platelet concentration improve Achilles tendon repair in rats. Journal of Acta Orthopedic scandandinavia, 2004;75(1):93-99.
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Bidder M, Toweler DA, Gelberman RH, Boyer MI. Expression of mRNA for vascular endothelial growth factor at the repair site of healing canine flexor tendon. Journal of Orthopedic research, 2000;18(2):147-52.
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Muto T, Kokubu T, Mifune Y, Inui A, Sakata R, Harada Y et al. Can platelet-rich plasma protect rat Achilles tendons from deleterious effects of triamcinolone acetonid? Orthopedic Journal of Sport Medicine, 2015;3(7):1-6.
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Dimitris NL, Konstantinos K, Dionysis V, Stotirios B, Georg A, Anna K. The effect of platelet-rich plasma gel in the early phase of patellar tendon healing. Journal of Archives of Orthopedic and Trauma Surgery, 2009; 129:1577-82.
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Tony WL, Luis C, Louis JS. Biomechanics of tendon injury and repair. Journal of Biomechanics, 2004;37;865-77.
36
Yuan T, Zhang CQ, Wang JH-C. Augmenting tendon and ligament repair with platelet-rich plasma(PRP). Muscle, Ligaments and Tendon Journal, 2013;3(3):139-49.
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Sen B, Guler S, Cecen B, Kumtepe E, Bagriyanik A, Ozkal S. The effect of autologous platelet rich plasma in the treatment of Achilles tendon ruptures: An experimental study on rabbits. Balkan Medical Journal, 2016;33(1):94-101.
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Dahners LE. Mechanical properties of tendons. In: Maffulli N, Renstrom P, Leadbetter WB editors. Tendon injuries: Basic science and clinical medicine. 1st ed. London: Springer 2005;13-21.
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Galloway MT, Lalley AL, Shearn JT. The role of mechanical loading in tendon development, maintenance, injury and repair. Journal of Bone& Joint Surgery, 2013; 95:1620-8.
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Dowling BA, Dart AJ. Mechanical and functional properties of the equine superficial digital flexor tendon. The Veterinary Journal, 2005; 170:184-192.
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Liu HY. In vivo evaluation of the stiffness of the patellar tendon. Presented for Ph.D., Chapel Hill. University of North Carolina. 2008.
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Hettrich CM, Beamer BS, Bedi A, Deland K, Deng XH, Ying Liang et al. The effect of rhPTH on the healing of tendon to bone in rat model. Journal of Orthopedic Research, 2012; 30:769-774
44
ORIGINAL_ARTICLE
Tramadol Vs. Meloxicam in Controlling Postoperative Pain in Dental Extractions in Cats
Objective- Evaluate analgesic effect of meloxicam and tramadol following dental extractions in cats. Design-Experimental studyAnimals-20 DSH cats who were diagnosed with 3rd or 4th stage of periodontal disease at their third mandibular premolar were entered the study in order to perform surgical dental extraction.Procedure-A blood sample was taken prior to surgery to assess the level of cortisol and CPK. General anesthesia performed using ketamine and diazepam (IV, 8.5 mg/kg+0.2 mg/kg) and inhalation of isoflurane following intubation. 3rd mandibular premolar extracted in all of the patients using surgical procedure. The cats were randomly selected into two groups of A receiving Meloxicam (IV, 0.2 mg/kg) or B, receiving tramadol (IV, 3 mg/kg) at the time of induction of anesthesia. The analgesics were continued after the surgery for 24 hours. The score of pain were recorded using UMPS and assessment of serum level of cortisol and CPK at 2, 4 and 24 hours after the surgery performed.Results-The highest score of pain was recorded at 4 hours after the surgery in both groups. Level of cortisol was significantly higher at 4 hours after the procedure in group B (P= 0.035). The increase in CPK was statistically significant at 2,4 and 24 hours after the surgery in group B when compared to group B (P<0.05).Conclusion and Clinical Relevance- It is concluded that although tramadol and meloxicam are both effective in reducing pain at early hours after the surgery, meloxicam is more effective to control pain after the first few hours.
https://www.ivsajournals.com/article_50824_569ff2c0b1da2602ebe03d0d1e6e8d1c.pdf
2017-10-01
49
54
10.22034/ivsa.2017.50824
Dental pain
Tramadol
Meloxicam
Cat
Azin
Tavakoli
azin.tavakoli@gmail.com
1
Islamic Azad University, Garmsar branch
LEAD_AUTHOR
Parham
Pahlavan
dr.parhampahlevan@gmail.com
2
Faculty of Veterinary Medicine, Garmsar branch, Islamic Azad University,Garmsar, Iran
AUTHOR
1.Becker D.E. Pain Management: Part 1: Managing Acute and Postoperative Dental Pain. Anesthesia Progress. 2010; 57(2): 67–79.
1
2.Ong KS, Tan JM. Preoperative intravenous tramadol versus ketorolac for preventing postoperative pain after third molar surgery. International Journal of Oral Maxillofacial Surgery. 2004; 33(3): 274-278.
2
3.Mascia P, Brown BR, Friedman S. Toothache of nonodontogenic origin: a case report. Journal of Endodontics. 2003;29:608-610
3
4.Hargreaves K, Abbott PV. Drugs for pain management in dentistry. Australian Dental Journal Suppl 2005;50:4.
4
5.Becker D. E., Phero J. C. Drug therapy in dental practice: nonopioid and opioid analgesics. Anesthesia Progress. 2005;52:140–149.
5
6.Collins M, Young I, Sweeney P, et al. The effect of tramadol on dento-alveolar surgical pain. British Journal of Oral Maxillofacial Surgery. 1997;35:54-58.
6
7.Doroschak AM, Bowles WR, Hargreaves KM. Evaluation of the combination of flurbiprofen and tramadol for management of endodontic pain. Journal of Endodontics. 1999;25:660-663.
7
8.Jung YS, Kim DK, Kim MK, Kim HJ, Cha IH, Lee EW. Onset of analgesia and analgesic efficacy of tramadol/acetaminophen and codeine/acetaminophen/ibuprofen in acute postoperative pain: a single-center, single-dose, randomized, active-controlled, parallelgroup study in a dental surgery pain model. Clinical Therapeutics. 2004;26:1037-1045
8
9.Kimmey M. B. Cardioprotective effects and gastrointestinal risks of aspirin: maintaining the delicate balance. American Journal of Medicine. 2004;117:72–78.
9
10.Maticic D, Stejskal M, Pecin M, Kreszinger M, Pirkic B, Vnuk D, Smolec O, Rumen- jak, V. Correlation of pain assessment parameters in dogs with cranial cruciate surgery. Veterinarski arhiv. 2010;80:597-609.
10
11.Firth AM, Haldane SL. Development of a scale to evaluate post operative pain. Journal of American Veterinary Medical Association. 1999, 214(5):651-9.
11
12.Khan AA, Dionne RA. The COX-2 inhibitors: new analgesic and anti-inflammatory drugs. Dental Clinics of North America. 2002;46(4):679-90.
12
13.Stein CS. The control of pain in peripheral tissue by opioids. The New England Journal of Medicine. 1995;332:1685–1690
13
14.Gopalraju P, Lalitha RM, Prasad K, Ranganath K. Comparative study of intravenous Tramadol versus Ketorolac for preventing postoperative pain after third molar surgery--a prospective randomized study. J of Craniomaxillofacial Surgery. 2014;42(5):629-33.
14
15.Feldsien JD, Wilke VL, Evans BR, Conzemius MG. (2010). Serum cortisol concentration and force plate analysis in the assessment of pain associated with sodium urate–induced acute synovitis in dogs. American Journal of Veterinary Research. 71(8): 940-945.
15
16.Grisneaux E, Pibarot P, Dupuis J, Blais D. Comparison of ketoprofen and carprofen administered prior to orthopedic surgery for control of postoperative pain in dogs. Journal of American Veterinary Medical Association. 1999;215(8): 1105-10.
16
17.Hancock RB, Lanz OI, Warldon DR, Duncan RB, Broadstone RV, Hendrix PK. Comparison of postoperative pain following ovariohysterectomy via harmonic scalpel assisted laparoscopy versus traditional celiotomy in dogs. Veterinary Surgery. 2005; 34: 273-282.
17
18.Arts M, Brand R, der Kallen BV, Nijeholt GL, Peul W. Does minimally invasive lumbar disc surgery result in less muscle injury than conventional surgery? A randomized controlled trial. European Spine Journal. 2011;20(1):51–57.
18
19.Kawaguchi Y, Matsui H, Tsuji H. Changes in serum creatine phosphokinase MM isoenzyme after lumbar spine surgery. The Spine Journal. 1997; 22:1018–1023.
19
20.Tavakoli A, Shafiee B, Mohammadyar L. Correlation of post-operative pain and levels of creatin phosphokinase enzyme following ovariohysterectomy in cats. Iranian Journal of Veterinary Medicine 2016;10(1):41-46.
20
21.Nekoofar MH, Sadeghipanah M, Dehpour AR. Evaluation of meloxicam (A cox-2 inhibitor) for management of postoperative endodontic pain: a double-blind placebo-controlled study. Journal of Endodontics. 2003;29:634-637..Graham GG, Scott KF. Mechanism of action of paracetamol. American Journal of Therapeutics. 2005; 12(1):46-55.
21
23.Barden J, Edwards JE, McQuay HJ, Andrew Moore R. Pain and analgesic response after third molar extraction and other postsurgical pain. Pain 2004; 107:86-90.
22
24.Isiordia-Espinoza MA, Sánchez-Prieto M, Tobías-Azúa F, Reyes-García JG.Pre-emptive analgesic effectiveness of meloxicam versus tramadol after mandibular third molar surgery: a pilot study.Journal of Oral Maxillofacial Surgery.2012;70(1):31-6.
23
25.Goodman LA, Brown SA, Torres BT, Reynolds LR, Budsberg SC. Effects of meloxicam on plasma iohexol clearance as a marker of glomerular filtration rate in conscious healthy cats. American Journal Veterinary Research. 2009;70(7):826-30.
24
26.Ingwersen W, Fox R, Cunningham G, and Winhall M. Efficacy and safety of 3 versus 5 days of meloxicam as an analgesic for feline onychectomy and sterilization. Canadian Veterinary Journal. 2012;53(3):257–264.
25
ORIGINAL_ARTICLE
A Histopathologic Study of Effects of Olive Oil Plus Lime Water on Third-Degree Burn in Mouse Model
Objective-The objective of this study was to compare effects of olive oil and lime water combination with silver sulfadiazine in third-degree burn healing.Design-Randomized experimental study.Animals-Sixty-three adult male Bulb/C mice weighing25±5 gr.Procedures-The mice were anesthetized with an intraperitoneal injection of ketamine 10% and xylazine 2% combination and the third-degree burn wound was created in the area of 1×1 cm at the dorsum of the animals using an innovated electrical device. There were three groups of 21 as follows: Group I–Negative control; which received the topical normal saline solution, Group II–Positive control; with the daily topical application of silver sulfadiazine ointment, and Group III–Treatment; which was received topical olive oil plus lime water, daily. Each group was divided into three subgroups and topical treatments or saline were applied to each subgroup for 7, 14, and 21 days, respectively. No other dressing was used. The mice of each subgroup were sacrificed on days 7, 14, and 21 and hematoxylin-eosin (H&E) stained slides were prepared. Histopathologic evaluations include epidermal thickness, secondary infection, and percentage of collagen, ground substance, fibroblast, and blood vessels.Results-Group II showed significantly less secondary infection, and secondary infection in group III was significantly reduced compared to group I. The epidermal thickness of group III had a significant difference with group II at 2nd week. Both group II and III were induced more collagen synthesis at 2nd week compared to group I. This was also true about ground substance. Group III had more angiogenesis at 2nd week compared to others, but ultimately this difference was diminished.Conclusions and Clinical Relevance-Despite lime water has some cytotoxic effects, combining with olive oil can reduce these unwanted effects. Thus, the combination may be beneficial in third-degree burn wounds in mice compared to routinely used silver sulfadiazine therapy.
https://www.ivsajournals.com/article_50916_706df4274a9226b31415b722a6307ead.pdf
2017-10-01
55
63
10.22034/ivsa.2017.50916
Lime water
Olive oil
silver sulfadiazine
Third-degree burn
Mouse
Siamak
Kazemi-Darabadi
s.kazemi@tabrizu.ac.ir
1
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
LEAD_AUTHOR
Ghasem
Akbari
g.akbari@tabrizu.ac.ir
2
Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
AUTHOR
Seyed-Hossein
Jarolmasjed
jarolmasjed@tabrizu.ac.ir
3
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
AUTHOR
Amir-Ali
Shahbazfar
shahbazfar@tabrizu.ac.ir
4
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
AUTHOR
Venter NG, Marques RG, dos Santos JS and Monte-Alto-Costa A. Use of platelet-rich plasma in deep second-and third-degree burns. Burns, 2016; 42(4):807-814.
1
Burn incidence and treatment in the United States: 2016 fact sheet. American Burn Association, 2016.
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Banati A, Chowdhury SR and Mazumder S. Topical use of sucralfate cream in second and third degree burns. Burns, 2001; 27(5):465-469.
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Akhoondinasab MR, Khodarahmi A, Akhoondinasab M, Saberi M and Iranpour M. Assessing effect of three herbal medicines in second and third degree burns in rats and comparison with silver sulfadiazine ointment. Burns, 2015; 41(1):125-131.
4
Akbari G, Shahbazfar A, Kianifard D, Rezaei H, Shokrollahi S and Mohebi D. Microscopic study of the healing effects of the mixture of olive oil and lime water on the second degree burning in rats. Journal of Ilam University of Medical Sciences, 2017; 24(6):169-177.
5
Sumer Z, Yildirim G, Sumer H and Yildirim S. Cytotoxic and antibacterial activity of the mixture of olive oil and lime cream in vitro conditions. African Journal of Traditional, Complementary and Alternative Medicines, 2013; 10(4):137-143.
6
Orey C. The healing powers of olive oil: a complete guide to nature's liquid gold. Healing Powers, 2015.
7
Dacosta ML and Regan MC. Diphenyl hydantoin sodium promotes early and marked angiogenesis and results in increased collagen deposition and tensile strength in healing wounds. Surgery, 1998; 123:287-293.
8
Gamilli RL. International society for burn injuries survey: infection and infectious complications in worldwide burn units. Burns, 2004; 30:655-659.
9
Kazemi-Darabadi S, Sarrafzadeh-Rezaei F, Farshid AA and Baradar-Jalili R. Healing of excisional wound in alloxan induced diabetic sheep: A planimetric and histopathologic study. Veterinary Research Forum, 2013; 4(3):149-155.
10
Tortora GJ and Grabowski SR. Measuring blood pressure. In: Principles of anatomy and physiology. 9th ed. New York; John Wiley & Sons Co, 2000; 690.
11
Hettiaratchy S and Papini R. ABC of burns: Initial management of a major burn: II—assessment and resuscitation. British Medical Journal, 2004; 329(7457):101.
12
Butcher M and Swales B. Assessment and management of patients with burns. Nursing Standard, 2012; 27(2):505-506.
13
Ramirez-Blanco CE, Ramirez-Rivero CE, Diaz-Martinez LA and Sosa-Avila LM. Infection in burn patients in a referral center in Colombia. Burns, 2017; 43(3):642-653.
14
Erol S, Altoparlak U, Akcay MN, Celebi F and Parlak M. Changes of microbial flora and wound colonization in burned patients. Burns, 2004; 30(4):357-361.
15
National burn repository 2012 report. American Burn Association; 2012.
16
Sherwood ER and Toliver-Kinsky T. Mechanisms of the inflammatory response. Best Practice & Research Clinical Anaesthesiology, 2004; 18(3):385–405.
17
Salas CL, Fernándes MM and Martínez DL. Topical chemotherapy for the treatment of burns. Revista de Enfermeria, 2005; 28(5):67-70.
18
Adhya A, Bain J, Ray O, Hazra A, Adhikari S, Dutta G, Ray S and Majumdar BK. Healing of burn wounds by topical treatment: A randomized controlled comparison between silver sulfadiazine and nano-crystalline silver. Journal of basic and clinical pharmacy, 2014; 6(1):29.
19
Gurfinkel R, Palivatkel-Naim M, Gleisinger R, Rosenberg L and Singer AJ. Comparison of purified olive oil and silver sulfadiazine in the treatment of partial thickness porcine burns. The American journal of emergency medicine, 2012; 30(1):79-83.
20
Rosen J, Landriscina A, Kutner A, Adler BL, Krausz AE, Nosanchuk JD and Friedman AJ. Silver sulfadiazine retards wound healing in mice via alterations in cytokine expression. The Journal of Investigative Dermatology, 2015; 135(5):1459.
21
Babich H and Visioli F. In vitro cytotoxicity to human cells in culture of some phenolics from olive oil. Il Farmaco, 2003; 58(5):403-407.
22
Motta G, Ratto GB, De Barbieri A, Corte G, Zardi L, Sacco A and Castagnola M. Can heterologous collagen enhance the granulation tissue growth? An experimental study. The Italian Journal of Surgical Sciences, 1983; 13(2):101-108.
23
Kıyan S, Uyanıkgil Y, Altuncı YA, Çavuşoğlu T, Uyanıkgil EÖ and Karabey F. Investigation of acute effects of Hypericum perforatum (St. John’s Wort-Kantaron) treatment in experimental thermal burns and comparison with silver sulfadiazine treatment. Ulus Travma Acil Cerrahi Derg, 2015; 21(5):323-236.
24
Singer AJ, Berrutti L, Thode HC and McClain SA. Octylcyanoacrylate for the treatment of partial‐thickness burns in swine: a randomized, controlled experiment. Academic Emergency Medicine, 1999; 6(7):688-692.
25
Tonnesen MG, Feng X and Clark RA. Angiogenesis in wound healing. Journal of Investigative Dermatology Symposium Proceedings 2000; 5(1):40-46.
26
Dimmeler S and Zeiher AM. Endothelial cell apoptosis in angiogenesis and vessel regression. Circulation Research. 2000; 87(6):434-439.
27
Reddy GK, Stehno‐Bittel L and Enwemeka CS. Matrix remodeling in healing rabbit Achilles tendon. Wound Repair and Regeneration, 1999; 7(6):518-527.
28
Lee AR and Moon HK. Effect of topically applied silver sulfadiazine on fibroblast cell proliferation and biomechanical properties of the wound. Archives of Pharmacal Research, 2003; 26(10):855-860.
29
Witte M and Barbul A. General principles of wound healing. Surgical Clinics of North America, 1997; 77(3):509-528.
30
Stroncek JD and Reichert WM. Overview of wound healing in different tissue types. In: Indwelling neural implants: strategies for contending with the in vivo environment. CRC Press/Taylor & Francis; 2008:3-40.
31
ORIGINAL_ARTICLE
Double Intestinal Intussusception due to Acute Enteritis in a Young Tibetan Spaniel Dog
AbstractCase Description A six-month-old female Tibetan spaniel dog with repeated rectal prolapse and unsuccessful treatments was referred to the clinic of faculty of veterinary medicine of Razi University (Kermanshah, Iran). With regarding the patient’s history colopexy was done through celiotomy incision, but 3 days later the patient referred again with recurrence of prolapse. Clinical FindingsOn abdominal palpation, a sausage like mass was felt in the abdomen. The clinical parameters were in the normal range, but stool samples proved the presence of giardia. The hemagglutination test for parvovirus was positive too. Treatment and Outcome Exploratory celiotomy revealed presence of double intussusception.The intussuscepted segments were edematous and congested with adhesions and signs of devitalization. Resection and re-anastomosis was performed. The patient died 24 hours after surgery. The owner didn’t allow post-mortem examination; though the actual cause of death was remained unknown. The animal death can be related to weakness due to parvovirus and giardia enteritis, delay in treatment of underlying disease, electrolyte imbalance, surgical stress and inadequate postoperative management. Clinical RelevancePuppies and kittens have a much higher incidence of intussusception than adult animals. Any portion of the alimentary tract may be involved, but previous studies have indicated that the majority of intussusceptions in small animal are enterocolic. Prompt and precise diagnosis and accurate treatment with considering underlying diseases such as infectious enteritis and endoparrasitism is very important to save the patient life.
https://www.ivsajournals.com/article_50242_5e70f828866c6b74bb75620c0ae69fe6.pdf
2017-10-01
64
68
10.22034/ivsa.2017.50242
double intussusception
Dog
celiotomy
Ali
Ghashghaii
aghashghaii@razi.ac.ir
1
Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran
LEAD_AUTHOR
Moosa
Javdani
javdani59@gmail.com
2
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
AUTHOR
Parisa
Mazdarani
p_mazdarani@yahoo.com
3
Department of Surgery and Radiology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran
AUTHOR
Fossom TW, Dewey CW, Radlinsky MAG, et al. Textbook of Small Animal Surgery. 4th ed, Elsevier St, Louis Mo USA, 2013;536-537.
1
Kumar V, Ahmad RA and Amarpal. Colopexy as a Treatment for Recurrent Rectal Prolapse in a Dog. Indian Journal of Canine Practice, 2012;4(2):138-140.Ghashghaii A. Correction of Recurrent Anorectal Prolapse in a 4 Months Dog by Colopexy Operation, in Proceedings. 6th Irannian Symposium of Veterinary Surgery, Anesthesia and Radiology, 2006; 43 (In Persian)
2
Hall EJ, German AJ. Disease of the small Intestine. In: Ettinger SJ, eds. Textbok of Veterinary Internal Medicine. 7th ed. Los Angeles, California: California Animal Hospital Veterinary Specialty Group, 2010;1571-1592.
3
Joy CL and Patterson JM. Short bowel syndrome following surgical correction of a double intussusception in a dog. Canadian Veterinary Journal, 1978;19:254-259.
4
Cina M, Rahim F and Davudi M. The Accuracy of Ultrasonography Technique in Detection of the Intussusception. Journal of Applied Sciences, 2009;9:3922-3926.
5
Gelberg HB. Alimentary System and the Peritoneum, Omentum, Mesentery, and Peritoneal Cavity. In: McGavin MD and Zachary JF, eds. Pathologic Basis of Veterinary Disease. 5th ed, Elsevier St, Louis Mo USA, 2012;363-364.
6
Levitt L and Bauer MS. Intussusception in dogs and cats: A review of 36 cases. Canadian Veterinary Journal, 1992;33:660-664.
7
Han TS, Kim JH, Cho K, et al. Double intussusceptions in a Shih-tzu puppy. Journal of Biomedical Research, 2008;9:55-58.
8
Valiei K and Beheshti R. Double Intussusception in Dog. Asian Journal of Animal and Veterinary Advances, 2011;6(9):971-976.
9
Wilson GP and Burt JK. Intussusception in the dog and cat: A review of 45 cases. Journal of the American Veterinary Medical Association, 1974;164:515-518.
10
Larsen LH and Bellenger CR. Stomach and Small Intestine. In: Archibald J, eds. Canine Surgery. 2nd ed. California: American Veterinary Publications, Santa Barbara, 1974;583-585.
11
Rosin E. Small intestinal surgical disorders. In: Slatter DJ, eds. Textbook of Small Animal Surgery. Vol 1. Toronto: W.B. Saunders, 1985;748-749.
12
Patsikas MN, Jakovljevic S, Moustardas N, et al. Ultrasonographic signs of intestinal intussusception associated with acute enteritis or gastroenteritis in 19 young dogs. Journal of the American Animal Hospital Association, 2003;39:57-66.
13
Butler HC. Surgery of the small intestine. Veterinary Clinics of North America: Small Animal Practice, 1972;2:160-161.
14
Lewis DD and Ellison GW. Intussusception in dogs and cats. Compendium on Continuing Education for the Practicing Veterinarian, 1987;9:523-534.
15
Hayden GE and Sprouse KL. Bowel obstruction and hernia. Emergency Medicine Clinics of North America, 2011;29:319-345.
16
Oakes MG, Lewis DD, Hosgood G, et al. Enteroplication for the prevention of intussusception recurrence in dogs: 31 cases (1978- 1992). Journal of the American Veterinary Medical Association, 1994;205:72-75.
17
Kumar V, Aijaz Ahmad R and Pathak R. Ileocolic Intussusception and its Surgical management in a Labrador Pup. Intas Polivet, 2012;13(1):108-110.
18
Sivasankar M. Recurrent intussusception in a 14-month old, spayed female German shepherd cross. Canadian Veterinary Journal, 2000;41:407-08.
19
Ellison GW. Nontraumatic Surgical Emergencies of the Abdomen. In: Red B, eds. Contemporary Issues in Small Animal Practice. Vol 2. New York: Livingstone, 1986;127-173.
20
Ellison GW. Intestinal Resection and anastomosis. In: Bojrab MJ, ed. Current Techniques in Small Animals Surgery. 5th eds. Philadelphia: Lea and Febiger, 2014;280-303.
21
ORIGINAL_ARTICLE
Unusual Case of Utero-Ovarian Prolapse Concurrent with Cystocele in a Queen
Case Description- A 1-year-old female Domestic Shorthair cat weighing 2.5 kg with one week history of protruding mass from the vulva was admitted.Clinical Findings- The prolapse was complete involving both horns protruding from the vulva and a soft bulging mass was palpable inside the prolapsed uterus.Treatment and Outcome- The prolapsed organ was irrigated with warm saline solution and the debris was cleaned. A ventral midline celiotomy was performed for reduction of the mass and sterilization of the cat. The urinary bladder was incarcerated in the right horn of the uterus. The left ovary was inside the mass beside the bladder. Ovarian pedicles were intact but broad ligament was torn. An ovariohysterectomy was performed.Clinical Relevance- Complete uterine prolapse is an emergency case of surgery. If the prolapse includes abdominal contents, amputation of the mass may be avoided and reduction of the uterus and abdominal contents through celiotomy should be prioritized. It seems that this case is the first report of an ovarian prolapse coincident with retroversion of the uterus. The prognosis following ovariohysterectomy is excellent if shock and hemorrhage are treated appropriately.
https://www.ivsajournals.com/article_51288_a09517be11acf78abc77f953f2946d5a.pdf
2017-10-01
69
73
10.22034/ivsa.2017.51288
utero-ovarian prolapse
cystocele
queen
Seyedhosein
Jarolmasjed
jarolmasjed@tabrizu.ac.ir
1
Department of clinical sciences, Faculty of veterinary medicine, University of Tabriz, Iran
LEAD_AUTHOR
1. Feldman EC and Nelson RW. Periparturient diseases. In: Feldman EC and Nelson RW, eds. Canine and feline endocrinology and reproduction. 3rd ed. St Louis, MO: Saunders, 2004;808.
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2. Stone EA. Ovary and uterus. In: Slatter DH, ed. Textbook of small animal surgery. 3rd ed. Philadelphia, PA: Saunders, 2003;1487.
2
3. Ekstrand C, Linde‐Forsberg C. Dystocia in the cat: a retrospective study of 155 cases. Journal of Small Animal Practice, 1994;35:459-464.
3
4. Wallace L, Henry JJ, Clifford J. Manual reduction of uterine prolapse in a domestic cat. Veterinary Medicine, Small Animal Clinician, 1970;65:595-596.
4
5. Murphy A, Dobson H. Predisposition, subsequent fertility, and mortality of cows with uterine prolapse. Veterinary Record, 2002;151:733-735.
5
6. Vanderhust S. Bicornuate uterine prolapse in a cat/a photographic essay. Veterinary Medicine, Small Animal Clinician, 1975;70:681.
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7. Maxson F, Krausnick K. Dystocia with uterine prolapse in a Siamese cat. Veterinary Medicine, Small Animal Clinician, 1969;64:1065-1066.
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8. Shumaila Y, Bushra H, Tabinda R. Extensive uterovaginal prolapse during labor. Journal of Obstetrics and Gynaecology Research, 2011;37:264-266.
8
9. Barber MD. Symptoms and outcome measures of pelvic organ prolapse. Clinical Obstetrics and Gynecology, 2005;48:648-661.
9
10. Bonney V. The treatment of ovarian prolapse by shortening the ovarian ligament. The Lancet, 1906;168:1717-1719.
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11. Fossum TW. Small animal surgery textbook. 4th ed. St. Louis, Missouri: Elsevier Health Sciences, 2013;826-827.
11
12. Miesner MD, Anderson DE. Management of uterine and vaginal prolapse in the bovine. Veterinary Clinics of North America: Food Animal Practice, 2008;24:409-419.
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13. Tobias KM, Johnston SA. Veterinary Surgery: Small Animal. 1st ed. St. Louis, Missouri: Elsevier Health Sciences, 2013;1886-1889.
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14. Holt P. Long-term evaluation of colposuspension in the treatment of urinary incontinence due to incompetence of the urethral sphincter mechanism in the bitch. Veterinary Record, 1990;127:537-542.
14