Iranian Journal of Veterinary Surgery

Iranian Journal of Veterinary Surgery

Chitosan Gel Containing Hypericum perforatum Extract Inhibiting Effect on Oxidative Stress in Burn Wounds of Rats

Document Type : Original Article

Authors
Pouria Khanzadeh 1
Maryam Hashemi 2, 3
Mohsen Maleki 4
Farzad Hayati 1
Ahmadreza Mohamadnia 1
1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
2 Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
3 Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
4 Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
10.30500/ivsa.2024.426741.1378
Abstract
The current study aimed to examine the impacts of topical sole and a combination of chitosan gel (Chit) and ethanolic extract (Ext) of Hypericum perforatum on inhibiting oxidative stress chain in burn wounds of Wistar rats. Three circular burn wounds on the back of the rats were induced in this study. The experimental groups included Sham, Chit, Silver Gel, Ext 5%, Ext 10%, Chit/Ext 5%, and Chit/Ext 10%. In addition to the evaluation of the healing process, total antioxidant capacity (TAC), malondialdehyde (MDA), and hydroxyproline levels were assessed. The results of the current study demonstrated that Chit/Ext treatment, especially at the 10% concentration, significantly reduced MDA, and remarkably enhanced TAC and hydroxyproline levels compared to the other groups. Based on the current study findings, it can be concluded that topical use of Chit and Ext can accelerate the healing process of second-degree burn wounds by inhibiting oxidative stress and inflammation and promoting angiogenesis and collagen production.
Keywords
Burn
Wound healing
Chitosan
Hypericum perforatum
Subjects
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  • Receive Date 24 November 2023
  • Revise Date 01 January 2024
  • Accept Date 08 January 2024
  • First Publish Date 08 January 2024