Iranian Journal of Veterinary Surgery

Iranian Journal of Veterinary Surgery

Effects of Interaperitoneal Administration of α-Tocopherol Acetate on Ischemia-Reperfusion Injury in Ovaries in Female Cats of Animal House Shelter Undergoing Elective Ovariohysterectomy

Document Type : Original Article

Authors
Abdolrahman Baghban 1
Rahim Mohammadi 1
Vahid Mohammadi 2
Shahram Javadi 2
1 Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
2 Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
10.30500/ivsa.2024.419864.1368
Abstract
Effects of interaperitoneal administration of α-tocopherol acetate on ischemia-reperfusion injury in ovaries torsion and detorsion were studied using a cat ovary model. Twenty healthy female DSH cats ~3 kg were randomized into five experimental groups (n = 5): Group SHAM: The cats underwent only celiotomy. Group Ischemia: A 3-hour ischemia only. Group I/R: A 3-hour ischemia and a 3-hour reperfusion. Group I/ATA: A 3-hour ischemia only and 10 mg/kg intraperitoneal administration (IP) of α-tocopherol 2.5 hours after induction of ischemia. Group I/R/ATA: A 3-hour ischemia, 3-hour reperfusion, and 10 mg/kg IP of α-tocopherol 2.5 hours after induction of ischemia. Animals treated with ATA showed significantly ameliorated development of ischemia and reperfusion tissue injury compared to those of other groups (p < 0.05). The significantly higher values of SOD, tGSH, GPO, GSHRd, and GST were observed in I/R/ATA animals compared to those of other groups (p = 0.001). Damage indicators (NOS, MDA, MPO, and DNA damage level) were significantly lower in I/R/ATA animals compared to those of other groups (p = 0.001). Intraperitoneal administration of ATA could be helpful in minimizing ischemia-reperfusion injury in ovarian tissue exposed to ischemia.
Keywords
Ischemia-reperfusion
α -tocopherol acetate
Intraperitoneal
Ovary
Subjects
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  • Receive Date 08 October 2023
  • Revise Date 06 February 2024
  • Accept Date 05 March 2024
  • First Publish Date 05 March 2024