Iranian Journal of Veterinary Surgery

Iranian Journal of Veterinary Surgery

The Effect of β-Cryptoxanthin on Testicular Ischemia-Reperfusion Injury in a Rat Model: Evidence from Testicular Histology

Document Type : Original Article

Authors
Kianoush Mohammadnejad 1
Rahim Mohammadi 1
Ali Soleimanzadeh 2
Ali Shalizar-Jalali 3
Farshid Sarrafzadeh-Rezaei 1
1 Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
2 Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
3 Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
10.30500/ivsa.2024.457134.1405
Abstract
Testicular torsion and detorsion are significant clinical issues for infertile man. Torsion of the spermatic cord is an emergency condition resulting from rotation of the testis and epididymis around the axis of the spermatic cord. A rat testis model was used to assess effects of β-cryptoxanthin on ischemia-reperfusion injury. Twenty healthy male Wistar rats were included and randomized into four investigational groups (n = 5): Group SHAM: In this group, midline incision of the scrotum was performed and the testicles were taken out for 2 hours with a 720-degree rotation. Group ISCHEMIA: In this group, midline incision of the scrotum was performed and the testicles were taken out and undergone ischemia for 2 hours with a 720-degree rotation. Group IS/REP/Oil: In this group, a midline scrotum cut was performed and the testicles were taken out and ischemia was created for 2 hours with a 720-degree rotation and at the end of ischemia 100 µl of corn oil (β-cryptoxanthin solvent) was injected intraperitoneally. Group IS/REP/CRPTXNTN 2.5: The same as group IS/REP/Oil as well as intraperitoneal administration of 100 µl of β-cryptoxanthin (2.5 µg/kg) at the end of ischemia. In all groups, the testes were returned back to scrotum and after 60 days were dissected out and removed for histopathological analyses. β-cryptoxanthin at the dose of 2.5 µg/kg significantly improved histologic indices compared to other treatment groups (p < 0.05). β-cryptoxanthin could be helpful in minimizing ischemia-reperfusion injury in testicular tissue exposed to ischemia.
Keywords
β-cryptoxanthin
Ischemia-reperfusion
Torsion
Testis
Subjects
  1. McLachlan RI, de Krester DM. Male infertility: the case for continued research. Medical Journal of Australia. 2001; 174(3): 116-117. doi: 10.5694/j.1326-5377.2001.tb143180.x
  2. Srinath H. Acute scrotal pain. Australian Family Physician. 2013; 42(11): 790-792.
  3. Arap MA, Vicentini FC, Cocuzza M, Hallak J, Athayde K, Lucon AM, Arap S, Srougi M. Late hormonal levels, semen parameters, and presence of antisperm antibodies in patients treated for testicular torsion. Journal of Andrology. 2007; 28(4): 528-532. doi: 10.5534/wjmh.190037
  4. Parhizkar P, Mohammadi R, Shahrooz R, Mohammadi V. Effects of pyrroloquinoline quinone (PQQ) on ischemia-reperfusion injury in rat ovaries: histological and biochemical assessments. Bulletin of Emergency and Trauma. 2019; 7(1): 35-40. doi: 10.29252/beat-070105
  5. Vakili-Sadeghi E, Najafpour A, Mohammadi R. Protective effects of propolis on ischemia-reperfusion injury in a rat testicular torsion and detorsion model. Veterinary Research Forum. 2023; 14(7): 389-395. doi: 10.30466/vrf.2023.1972630.3682
  6. Girotti AW. Lipid hydroperoxide generation, turnover, and effector action in biological systems. Journal of Lipid Research. 1998; 39(8): 1529-1542.
  7. Huang HY, Helzlsouer KJ, Appel LJ. The effects of vitamin C and vitamin E on oxidative DNA damage: results from a randomized controlled trial. Cancer Epidemiology, Biomarkers and Prevention. 2000; 9(7): 647-652.
  8. Ames BN, Shigenaga MK, Hagen TM. Oxidants, antioxidants, and the degenerative diseases of aging. Proceedings of the National Academy of Sciences of the USA. 1993; 90(17): 7915-7922. doi: 10.1073/pnas.90.17.7915
  9. Raisi A, Davoodi F, Mohammadi R. Protective effects of rosmarinic acid on testicular torsion-detorsion in an animal model. Iranian Journal of Veterinary Surgery. 2022; 17(2); 80-86. doi: 10.30500/IVSA.2022.327175.1293
  10. Kazemi-Darabadi S, Asadpour R. Shahbazfar AA, Alizadeh S. Effects of L-carnitine and betamethasone on ischemia-reperfusion injuries and sperm parameters following testicular torsion in a rat model. Veterinary Research Forum. 2019; 10(2): 125-132. doi: 10.30466/vrf.2019.74767.2002
  11. Sarlak M, Roumiani E, Kheradmand A, Dezfoulian O, Alirezaei M, Raisi A, Davoodi, F. Evaluating the effects of betaine on testicular ischemia/reperfusion injury induced by torsion/detorsion in the rat. Andrologia. 2022; 54(10): e14559. doi: 10.1111/and.14559
  12. Burri B J, La Frano MR, Zhu C. Absorption, metabolism, and functions of β-cryptoxanthin. Nutrition Reviews. 2016: 74(2), 69–82. doi: 10.1093/nutrit/nuv064
  13. Azqueta A, Collins AR. Carotenoids and DNA damage. Mutation Research. 2012; 733(1-2): 4–13. doi: 10.1016/j.mrfmmm.2012.03.005
  14. Animal euthanasia policy. Approved and adopted. [Accessed May 03, 2023]. Available at: https://research.wustl.edu/wp-content/uploads/2017/05/Euthanasia.pdf.
  15. Ahmad MB, Tay MY, Shameli K, Hussein MZ, Lim JJ. Green synthesis and characterization of silver/chitosan/polyethylene glycol nanocomposites without any reducing agent. International Journal of Molecular Sciences. 2011; 12(8): 4872-4884. doi: 10.3390/ijms12084872
  16. Cosentino MJ, Nishida M, Rabinowitz R, Cockett AT. Histopathology of prepubertal rat testes subjected to various durations of spermatic cord torsion. Journal of Andrology. 1986; 7(1): 23-31. doi: 10.1002/j.1939-4640.1986.tb00862.x
  17. Cummings JM, Boullier JA, Sekhon D, Bose K. Adult testicular torsion. Journal of Urology. 2002; 167(5): 2109-2110.
  18. Aktaş BK, Bulut Ş, Bulut S, Baykam MM, Özden C, Şenes M, Yücel D, Memiş A. The effects of N-acetylcysteine on testicular damage in experimental testicular ischemia/reperfusion injury. Pediatric Surgery Internationa 2010; 26(3): 293-298. doi: 10.1007/s00383-009-2538-0
  19. Lee JW, Kim JI, Lee YA, Lee DH, Song CS, Cho YJ, Han JS. Inhaled hydrogen gas therapy for prevention of testicular ischemia/reperfusion injury in rats. Journal of Pediatric Surgery. 2012; 47(4): 736-742. doi: 10.1016/j.jpedsurg.2011.09.035
  20. Barlas M, Hatiboglu C. The effect of nitric oxide in testicular ischemia-reperfusion injury. International Urology and Nephrology. 2002; 34(1): 81-86. doi: 10.1023/a:1021311029572
  21. Wang WW, Qiao SY, Li DF. Amino acids and gut function. Amino Acids. 2009; 37(1): 105-110. doi: 10.1007/s00726-008-0152-4
  22. Vitaglione P, Ottanelli B, Milani S, Morisco F, Caporaso N, Fogliano V. Dietary trans-resveratrol bioavailability and effect on CCl4-induced liver lipid peroxidation. Journal of Gastroenterology and Hepatology. 2009; 24(4): 618-622. doi: 10.1111/j.1440-1746.2008.05598.x
  23. Ünsal A, Eroglu M, Avci A, Cimentepe E, Guven C, Derya Balbay M, Durak I. Protective role of natural antioxidant supplementation on testicular tissue after testicular torsion and detorsion. Scandinavian Journal of Urology and Nephrology. 2006; 40(1): 17-22. doi: 10.1080/00365590500407514
  24. Cuzzocrea S, Riley DP, Caputi AP, Salvemini,D. Antioxidant therapy: a new pharmacological approach in shock, inflammation, and ischemia/reperfusion injury. Pharmacological Reviews. 2001; 53(1): 135-159.
  25. Turner TT, Bang HJ, Lysiak JL. The molecular pathology of experimental testicular torsion suggests adjunct therapy to surgical repair. Journal of Urology. 2004; 172(6 Part 2): 2574-2578. doi: 10.1097/01.ju.0000144203.30718.19
  26. Jung JE, Kim GS, Chen H, Maier CM, Narasimhan P, Song YS, Song YS, Niizuma K, Katsu M, Okami N, Yoshioka H, Sakata H, Goeders CE, Chan PH. Reperfusion and neurovascular dysfunction in stroke: from basic mechanisms to potential strategies for neuroprotection. Molecular Neurobiology. 2010; 41(2-3): 172-179. doi: 10.1007/s12035-010-8102-z
  27. Abdel-Gaber SA, Mohammed RK, Refaie MM. Mechanism mediating the protective effect of diacerein in ischemia-reperfusion-induced testicular injury in rats. Life Sciences. 2018; 209: 57-62. doi: 10.1016/j.lfs.2018.07.060
  28. Cutrìn JC, Boveris A, Zingaro B, Corvetti G, Poli G. In situ determination by surface chemiluminescence of temporal relationships between evolving warm ischemia-reperfusion injury in rat liver and phagocyte activation and recruitment. Hepatology. 2000; 31(3): 622-632. doi: 10.1002/hep.510310312
  29. Kim YH, Kim GH, Shin JH, Kim KS, Lim JS. Effect of Korean red ginseng on testicular tissue injury after torsion and detorsion. Korean Journal of Urology. 2010; 51(11): 794-799. doi: 10.4111/kju.2010.51.11.794
  30. Celik O, Turkoz Y, Hascalik S, Hascalik M, Cigremis Y, Mizrak B, Yologlu S. The protective effect of caffeic acid phenethyl ester on ischemia-reperfusion injury in rat ovary. European Journal of Obstetrics, Gynecology, and Reproductive Biology. 2004; 117(2): 183-188. doi: 10.1016/j.ejogrb.2004.05.007
  31. Zhang F, Shi D, Wang X, Zhang Y, Duan W, Li Y. β-cryptoxanthin alleviates myocardial ischaemia/reperfusion injury by inhibiting NF-κB-mediated inflammatory signalling in rats. Archives of Physiology and Biochemistry, 2022; 128(4): 1128–1135. doi: 10.1080/13813455.2020.1760302
  32. Codo˜ner-Franch P, Mu˜niz P, Gasco E, Domingo JV, Valls-Belles V. Effect of a diet supplemented with alpha-tocopherol and b-carotene on atp and antioxidant levels after hepatic ischemia-reperfusion. Journal of Clinical Biochemistry and Nutrition. 2008; 43: 13-18. doi: 10.3164/jcbn.2008038
  33. Vardi N, Parlakpinar H, Ozturk F, Ates B, Gul M, Cetin A, Erdogan A, Otlu A. Potent protective effect of apricot and β-carotene on methotrexate-induced intestinal oxidative damage in rats. Food and Chemical Toxicology. 2008; 46: 3015-3022. doi: 10.1016/j.fct.2008.05.039
  34. Seyyed Ali M, Neisi N, Darbor M, Hassanpour M, Makvandi M, Solgi G. β-carotene protects the gastric mucosa against ischemia-reperfusion injury in rats. European Cytokine Network. 2012; 23(4): 173–178. doi: 10.1684/ecn.2012.0317
Iranian Journal of Veterinary Surgery
Volume 20, Issue 2 - Serial Number 43
October 2025
Pages 110-115

  • Receive Date 12 May 2024
  • Revise Date 29 July 2024
  • Accept Date 12 August 2024
  • First Publish Date 12 August 2024